Validation Data Gallery
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Tested Applications
| Positive WB detected in | fetal human brain tissue, pig brain tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
66253-1-Ig targets GNAS in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GNAS fusion protein Ag20530 相同性解析による交差性が予測される生物種 |
| Full Name | GNAS complex locus |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC002722 |
| Gene Symbol | GNAS |
| Gene ID (NCBI) | 2778 |
| RRID | AB_2881641 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95467 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GNAS antibody 66253-1-Ig | Download protocol |
| IHC protocol for GNAS antibody 66253-1-Ig | Download protocol |
| IF protocol for GNAS antibody 66253-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Vet Microbiol Porcine circovirus-like virus P1 activates pancreatic secretion pathway by interacting with CHRM3 protein | ||
FASEB J GRP78 acts as a cAMP/PKA signaling modulator through the MC4R pathway in porcine embryonic development | ||
iScience Bitter taste receptor T2R14-Gαi coupling mediates innate immune responses to microbial quorum sensing molecules in cystic fibrosis |