Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-83988-4 targets GDF5 in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | GDF5 fusion protein Ag6238 相同性解析による交差性が予測される生物種 |
| Full Name | growth differentiation factor 5 |
| Calculated molecular weight | 55 kDa |
| GenBank accession number | BC032495 |
| Gene Symbol | GDF5 |
| Gene ID (NCBI) | 8200 |
| RRID | AB_3673332 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43026 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Growth and Differentiation Factor 5 (GDF5) plays important roles during joint formation. It is one of the earliest genes expressed in the embryonic joint interzone7-10, fated to give rise to joint tissues including articular cartilage, synovium, menisci, and ligaments.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GDF5 antibody CL488-83988-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |