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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using GDF5 Recombinant antibody (83988-4-RR)

    IF Staining of RAW 264.7 using 83988-4-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using GDF5 antibody (83988-4-RR, Clone: 240944E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83988-4-RR

    Affinity and Kinetic Characterization of 83988-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83988-4-RR against Human GDF5 were performed. The affinity constant is 4.07 nM.

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GDF5 Recombinant antibody

GDF5 Recombinant Antibody for IF/ICC, ELISA
Cat No. 83988-4-RR
Clone No.240944E5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, ELISA

Synonyms

GDF-5, GDF 5, CDMP-1, Cartilage-derived morphogenetic protein 1, Bone morphogenetic protein 14

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83988-4-RR targets GDF5 in IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GDF5 fusion protein Ag6238 相同性解析による交差性が予測される生物種
Full Name growth differentiation factor 5
Calculated molecular weight 55 kDa
GenBank accession numberBC032495
Gene Symbol GDF5
Gene ID (NCBI) 8200
RRIDAB_3671563
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP43026
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Growth and Differentiation Factor 5 (GDF5) plays important roles during joint formation. It is one of the earliest genes expressed in the embryonic joint interzone7-10, fated to give rise to joint tissues including articular cartilage, synovium, menisci, and ligaments.

Protocols

Product Specific Protocols
IF protocol for GDF5 antibody 83988-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols