|Positive WB detected in||HeLa cells, HepG2 cells, HEK-293 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Sample-dependent, check data in validation data gallery|
HRP-60004 targets GAPDH in WB, IHC, IF applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.
|Tested Reactivity||human, mouse, rat, zebrafish, plant|
|Cited Reactivity||Chicken, hamster, Honey bees, human, mouse, opossum, pig, rabbit, rat|
|Host / Isotype||Mouse / IgG2b|
|Full Name||glyceraldehyde-3-phosphate dehydrogenase|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC004109|
|Gene ID (NCBI)||2597|
|Conjugate||HRP Fluorescent Dye|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hala (Verified Customer) (02-25-2022)
it works very good but i got an aspecific band around 30KDa
Laura (Verified Customer) (01-10-2020)
Benjamin (Verified Customer) (01-08-2020)
Works really well with high sensitivity and removes the need for a secondary. Needs to be dilute as GAPDH bands will be too intense at higher dilutions.
Kathryn (Verified Customer) (12-10-2019)
Used at 1:5000 in 5% BSA on a small amount of neonatal mouse ovary and worked beautifully.