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  • KD/KO Validated

GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, yeast, plant, zebrafish, pig and More (8)

Applications

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Conjugate

Unconjugated

CloneNo.

1E6D9

Cat no : 60004-1-Ig

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, ROS1728 cells, pig brain tissue, zebrafish tissue, whole yeast, whole Nematode tissue, soybean whole plant tissue, arabidopsis whole plant tissue, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, C6 cells, PC-12 cells, C2C12 cells, SP2/0 cells, rat brain tissue, mouse brain tissue
Positive IP detected inHeLa Cells
Positive IF detected inEthacrynic acid treated HeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:50000-1:500000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

60004-1-Ig targets GAPDH in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat, yeast, plant, zebrafish, pig samples.

Tested Reactivity human, mouse, rat, yeast, plant, zebrafish, pig
Cited Reactivity Deer, human, Goat, tick, treeshrew, Cynomorium songaricum, chicken, whiteflies, Yeast, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GAPDH fusion protein Ag0766 相同性解析による交差性が予測される生物種
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
Gene ID (NCBI) 2597
RRIDAB_2107436
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the molecular weight of GAPDH?

The molecular weight of GAPDH is 36 kD.


What is GAPDH?

GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" 

protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic 

production of ATP and pyruvate (PMID: 21895736).


What is the function of GAPDH?

GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even 

critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH 

is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting 

as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in 

transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 

11035021).


Where in the cell is GAPDH localized?

GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus 

(PMIDs: 21895736, 12829261).


Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear 

functions?

Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is 

useful as a control for Western blots and RT-PCR.


What are the known protein-protein interactions of GAPDH?

GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of 

these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).


Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.


Protocols

Product Specific Protocols
WB protocol for GAPDH antibody 60004-1-IgDownload protocol
IF protocol for GAPDH antibody 60004-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

A signalling pathway for transcriptional regulation of sleep amount in mice

Authors - Rui Zhou
humanWB

Nature

Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds.

Authors - Zhaoyang Li
humanWB

Nature

Structure of LRRK2 in Parkinson's disease and model for microtubule interaction.

Authors - C K Deniston
humanWB

Nature

The molecular basis of tight nuclear tethering and inactivation of cGAS.

Authors - Baoyu Zhao
mouse,humanWB

Nature

Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer.

Authors - Xinjian Liu
WB

Nature

A phase-separated nuclear GBPL circuit controls immunity in plants.

Authors - Shuai Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sammy (Verified Customer) (01-20-2024)

Excellent House Keeper. Diluted in 3% BSA PBS Tween (0.2%).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 4000
  • Cell Tissue Type: HUVEC
GAPDH Antibody Western Blot validation (1 in 4000 dilution) in HUVEC (Cat no:60004-1-Ig)
FH

Kamal (Verified Customer) (08-30-2023)

Works well with when diluted in 1X TBST.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Liver tissue
FH

Kamal (Verified Customer) (08-30-2023)

Works well with when diluted in 1X TBST.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Liver tissue
FH

Parijat (Verified Customer) (08-21-2023)

Works well, no backgrounds

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK-293T
FH

Amy (Verified Customer) (08-07-2023)

Good clean bands for WB of HEK293T cell lysates.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:20,000
  • Cell Tissue Type: HEK293T
FH

Mohamad (Verified Customer) (07-03-2023)

very good antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
FH

Angie (Verified Customer) (06-02-2023)

Protein lysates from A549 cells were subjected to SDS-PAGE followed by immunoblotting with 60004-1-Ig (mouse anti-GAPDH antibody) at dilution of 1:80 000 incubated at room temperature for 45 minutes. Secondary antibody: Donkey-anti-mouse (Alexa Fluor 680) at 1:20000 dilution incubated at room temperature for 1 hour .

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:80000
  • Cell Tissue Type: Human lung cancer cell A549
GAPDH Antibody Western Blot validation (1:80000 dilution) in Human lung cancer cell A549 (Cat no:60004-1-Ig)
FH

Udesh (Verified Customer) (09-27-2022)

The antibody was specific and detected a band close to 35 kD in BM-MSCs at 1:5000 dilution, overnight at 4 degrees.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Bone Marrow- MSCs
GAPDH Antibody Western Blot validation (1:5000 dilution) in Bone Marrow- MSCs (Cat no:60004-1-Ig)
FH

Janelle (Verified Customer) (09-12-2022)

Specific to target

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Yuki (Verified Customer) (08-23-2022)

A nice antibody to detect GAPDH in 293T cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:50000
  • Cell Tissue Type: 293T
GAPDH Antibody Western Blot validation (1:50000 dilution) in 293T (Cat no:60004-1-Ig)
FH

Ralf (Verified Customer) (08-04-2022)

10µg of total mouse protein were loaded on the gel. Excellent antibodies!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
GAPDH Antibody Western Blot validation (1:3000 dilution) in (Cat no:60004-1-Ig)
FH

Tsimafei (Verified Customer) (07-15-2022)

30µg of total protein were loaded on the gel. Antibodies were incubated ON at 4C.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: mESCs
GAPDH Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:60004-1-Ig)
FH

Xin (Verified Customer) (04-16-2022)

A nice antibody to detect GAPDH in both mouse cortex lysates.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse cortex lysate
GAPDH Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:60004-1-Ig)
FH

Shu Jun (Verified Customer) (04-14-2022)

Not applicable

  • Applications: Western Blot
  • Cell Tissue Type: HeLa cell
FH

Weihao (Verified Customer) (04-11-2022)

Great antibody! Clear band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000-10000
  • Cell Tissue Type: mouse BMDM
FH

Charlotte (Verified Customer) (03-09-2022)

Membrane was blocked for 1 hour with 5% nonfat milk in TBS + 0.01% Tween, then probed overnight at 4C with a 1:20,000 dilution of a-GAPDH antibody in blocking buffer. After washing, the membrane was probed with an a-mouse secondary antibody at 1:50,000 in TBST. Under these conditions, the signal is very robust, even at a short exposure (~30 seconds) and has low background. The GAPDH antibody could likely be used at lower concentrations. Quite pleased with how it looks!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:20000
  • Cell Tissue Type: Xenopus Embryo Extract
GAPDH Antibody Western Blot validation (1:20000 dilution) in Xenopus Embryo Extract (Cat no:60004-1-Ig)
FH

Uthra (Verified Customer) (03-03-2022)

Excellent antibody! It gives clean and specific results

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T
GAPDH Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:60004-1-Ig)
FH

Jinwei (Verified Customer) (08-31-2021)

This GAPDH Monoclonal antibody Cat No. 60004-1-Ig works OK.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293
GAPDH Antibody Western Blot validation (1:5000 dilution) in HEK293 (Cat no:60004-1-Ig)
FH

IMRAN AKHTAR (Verified Customer) (04-15-2021)

This antibody is very good. We have used it many times and with really good results.

  • Applications: Western Blot, Cell culture
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovarian cancer stem cells,
FH

Pradeep (Verified Customer) (02-02-2021)

Good antibody for WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: Mouse Heart
FH

Yin (Verified Customer) (01-27-2021)

293T lysate (20 ug) was loaded on 4-12% Bis-Tris gels and transferred to PVDF membrane. Membrane was incubated in 5% BSA in PBS/0.1% Tween-20 for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:5000) at 4 degree overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking buffer (1:10000) for 1h at room temperature. Membrane was developed in ECL and imaged on a Biorad chemiluminescence detection system.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: 293T
GAPDH Antibody Western Blot validation (1:5000 dilution) in 293T (Cat no:60004-1-Ig)
FH

Eiko (Verified Customer) (10-16-2020)

This antibody worked quite well in my western blotting. I used 5% skim milk TBS-T for blocking and antibody dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: hTERT-RPE1
GAPDH Antibody Western Blot validation (1:2000 dilution) in hTERT-RPE1 (Cat no:60004-1-Ig)
FH

Tom (Verified Customer) (09-28-2020)

HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:5000 in block) overnight at 4 degrees. Goat anti-mouse HRP (1:10,000) for 1 hour in block.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293T
GAPDH Antibody Western Blot validation (1:5000 dilution) in HEK293T (Cat no:60004-1-Ig)
FH

Federica (Verified Customer) (06-08-2020)

Excellent antibody.I used in WB on HeLa lysate and the results was extremely clear. No unspecific bands detected. Perfect as loading control.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: HeLa cells
GAPDH Antibody Western Blot, validation (1:10000 dilution) in HeLa cells (Cat no:60004-1-Ig)
FH

Alinda (Verified Customer) (01-27-2020)

Good antibody for Western BLOT. antibody diluted in 5%milk.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK 293T
GAPDH Antibody Western Blot, validation (1:1000 dilution) in HEK 293T (Cat no:60004-1-Ig)
FH

Aamir (Verified Customer) (01-20-2020)

Good signal for WB

  • Applications: Western Blot,
  • Cell Tissue Type: HEKs
FH

David (Verified Customer) (01-14-2020)

Excellent antibody. Single band at the correct molecular weight. No background and can be used at high dilutions.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: SH-SY5Y
FH

saswati (Verified Customer) (01-08-2020)

Need a 1 hr block with 5% milk / TBST as usual.1 hr room temp with the same blocking media with a 1:8000 dilution, followed by secondary anti mouse ( same dilution) , HRP conjugated 1 hr room temp.Detect and document.4 is rating , since a 1:8000 fold is tad bit low, have used better dilution ( 1:100,000) in the past, however that product is not available these days

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1 to 8000
  • Cell Tissue Type: MDA-MB-231
FH

Emma (Verified Customer) (12-23-2019)

This antibody works well detecting GAPDH in 3t3-L1 and primary mouse embryonic fibroblasts.

  • Applications: Western Blot,
  • Cell Tissue Type: 3t3-L1, pMEF
FH

Nhu Quynh (Verified Customer) (12-16-2019)

High afffinity and specific antibody. Useful for protein controls in western blots.

  • Applications: Western Blot,
  • Cell Tissue Type: Cardiomyocytes
FH

Jia (Verified Customer) (11-12-2019)

This antibody works really well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: CHO-K1 cell
GAPDH Antibody Western Blot, validation (1:1000 dilution) in CHO-K1 cell (Cat no:60004-1-Ig)
FH

Juan (Verified Customer) (10-20-2019)

WB, it works well in 1:10000 dilution.

  • Applications: Western Blot,
FH

Iram (Verified Customer) (10-01-2019)

Working excellent

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Pancreatic and Colon cancer cells
GAPDH Antibody Western Blot, validation (1:5000 dilution) in Pancreatic and Colon cancer cells (Cat no:60004-1-Ig)
FH

RICHA (Verified Customer) (09-26-2019)

Excellent antibody. Clean and specific

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:25000
  • Cell Tissue Type: Protein from mouse tail tendon
GAPDH Antibody Western Blot, validation (1:25000 dilution) in Protein from mouse tail tendon (Cat no:60004-1-Ig)
FH

Pau (Verified Customer) (09-02-2019)

Excellent and clean Western blots.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293T
FH

Tanusree (Verified Customer) (08-14-2019)

The antibody works good in mouse brain tissue in western blotting.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

Praveen (Verified Customer) (04-15-2019)

Good

  • Applications: Western Blot,
  • Cell Tissue Type: Ac16
GAPDH Antibody Western Blot, validation ( dilution) in Ac16 (Cat no:60004-1-Ig)
FH

Shubham (Verified Customer) (04-15-2019)

Very good

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
GAPDH Antibody Western Blot, validation ( dilution) in AC16 (Cat no:60004-1-Ig)
FH

Vanessa (Verified Customer) (04-14-2019)

Total cell lysate (5-20 ug) was resolved on 4-12% Bis-Tris gels and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% BSA in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:5000) at 4C overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking bufffer (1:10000) for 2h at room temperature. Membrane was developed in ECF reagent and imaged on a chemiluminescence detection system.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: brain
FH

Damaris (Verified Customer) (04-03-2019)

Excellent antibody! It gives clean and specific results

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: brain lysate
GAPDH Antibody Western Blot, validation (1:1000 dilution) in brain lysate (Cat no:60004-1-Ig)
FH

JIMMY (Verified Customer) (03-26-2019)

This antibody offers a good detection of GAPDH on mouse cells such as macrophages and microglia through western blots.

  • Applications: Western Blot,
FH

Elisabeth (Verified Customer) (02-05-2019)

Total cell lysate (15 ug) was resolved on 4-12% Bis-Tris gels and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-GAPDH in blocking buffer (1:10,000) at 4C overnight. After washing, membrane was incubated in anti-mouse-HRP in blocking bufffer (1:5000) for 2h at room temperature. Membrane was developed in ECL reagent and imaged on a chemiluminescence detection system.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 10,000
  • Cell Tissue Type: HeLa cells lysate
GAPDH Antibody Western Blot, validation (10,000 dilution) in HeLa cells lysate (Cat no:60004-1-Ig)
FH

Stephen (Verified Customer) (01-31-2019)

great antibody for WB as a loading control used 1:20000

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:20000
  • Cell Tissue Type: HeLa
GAPDH Antibody Western Blot, validation (1:20000 dilution) in HeLa (Cat no:60004-1-Ig)
FH

Kishor (Verified Customer) (01-30-2019)

I working good for Western Blotting (1:5000)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: H69, HUCCT-1
GAPDH Antibody Western Blot, validation (1:5000 dilution) in H69, HUCCT-1 (Cat no:60004-1-Ig)
FH

Wei (Verified Customer) (12-04-2018)

In muscle cells, GAPDH antibody shows two bands while in cancer cells there is only one band

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12
FH

Robert (Verified Customer) (12-03-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: neuronal cell culture
FH

Guillaume (Verified Customer) (11-21-2018)

This antibody is useful as a cytoplasmic control for cyto-nuclear extractions.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Prostate cancer cells
GAPDH Antibody Western Blot, validation (1/1000 dilution) in Prostate cancer cells (Cat no:60004-1-Ig)
FH

Ruth (Verified Customer) (11-21-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:40000
  • Cell Tissue Type: Human cell line lysates
FH

Tingting (Verified Customer) (11-20-2018)

The bands of the GADPH looked very good! The antibody used in trophoblast cell and diluted in 1:1000.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Trophoblast
FH

marian (Verified Customer) (11-20-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon
GAPDH Antibody Western Blot, validation (1:1000 dilution) in colon (Cat no:60004-1-Ig)
FH

vanessa (Verified Customer) (11-20-2018)

very efficient product and great customer service

  • Applications: Cell culture,
FH

Vermont (Verified Customer) (11-20-2018)

great sensitivity and reproducibility

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: macrophage
GAPDH Antibody Western Blot, validation (1:5000 dilution) in macrophage (Cat no:60004-1-Ig)
FH

Saswati (Verified Customer) (11-20-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:8000
  • Cell Tissue Type: Human breast cancer
FH

Qunfeng (Verified Customer) (01-11-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Huh7 cells
FH

Sinu (Verified Customer) (01-10-2018)

Worked well in the western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human THP1