• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GAPDH Monoclonal antibody (60004-1-Ig)

    WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GAPDH Monoclonal antibody (60004-1-Ig)

    WB analysis of HepG2 using 60004-1-Ig (same clone as 60004-1-PBS)

    Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a dilution of 1:10000. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GAPDH Monoclonal antibody (60004-1-Ig)

    WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GAPDH Monoclonal antibody (60004-1-Ig)

    WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of arabidopsis whole plant tissue using GAPDH Monoclonal antibody (60004-1-Ig)

    WB analysis of arabidopsis whole plant using 60004-1-Ig (same clone as 60004-1-PBS)

    arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GAPDH Monoclonal antibody (60004-1-Ig)

    WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of whole Nematode tissue using GAPDH Monoclonal antibody (60004-1-Ig)

    WB analysis of whole Nematode using 60004-1-Ig (same clone as 60004-1-PBS)

    whole Nematode tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of soybean whole plant tissue using GAPDH Monoclonal antibody (60004-1-Ig)

    WB analysis of soybean whole plant using 60004-1-Ig (same clone as 60004-1-PBS)

    soybean whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of zebrafish tissue using GAPDH Monoclonal antibody (60004-1-Ig)

    WB analysis of zebrafish using 60004-1-Ig (same clone as 60004-1-PBS)

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of whole yeast using GAPDH Monoclonal antibody (60004-1-Ig)

    WB analysis of whole yeast using 60004-1-Ig (same clone as 60004-1-PBS)

    whole yeast cells were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GAPDH Monoclonal antibody (60004-1-Ig)

    WB analysis of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

    Western blot of Hela cell with anti-GAPDH (60004-1-Ig) at various dilutions. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa Cells using GAPDH Monoclonal antibody (60004-1-Ig)

    IP experiment of HeLa Cells using 60004-1-Ig (same clone as 60004-1-PBS)

    IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GAPDH Monoclonal antibody (60004-1-Ig)

    IHC staining of human liver cancer using 60004-1-Ig (same clone as 60004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60004-1-Ig (GAPDH antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GAPDH Monoclonal antibody (60004-1-Ig)

    IHC staining of human liver cancer using 60004-1-Ig (same clone as 60004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60004-1-Ig (GAPDH antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GAPDH Monoclonal antibody (60004-1-Ig)

    IF Staining of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH antibody (60004-1-Ig, Clone: 1E6D9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using GAPDH Monoclonal antibody (60004-1-Ig)

    FC experiment of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GAPDH (60004-1-Ig, Clone:1E6D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

［PR］論文掲載5,600報突破！GFP免疫沈降用抗体「GFP-Trap®」を無料体験

  • Featured Product
  • KD/KO Validated

GAPDH Monoclonal antibody, PBS Only

GAPDH Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 60004-1-PBS
Clone No.1E6D9
Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, zebrafish, yeast, plant

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

1E6D9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Published Applications

WBSee 1 publications below

Product Information

60004-1-PBS targets GAPDH in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, zebrafish, yeast, plant samples.

Tested Reactivity human, mouse, rat, pig, zebrafish, yeast, plant
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GAPDH fusion protein Ag0766 相同性解析による交差性が予測される生物種
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene Symbol GAPDH
Gene ID (NCBI) 2597
RRIDAB_2107436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04406
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

What is the molecular weight of GAPDH?

The molecular weight of GAPDH is 36 kD.


What is GAPDH?

GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" 

protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic 

production of ATP and pyruvate (PMID: 21895736).


What is the function of GAPDH?

GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even 

critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH 

is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting 

as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in 

transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 

11035021).


Where in the cell is GAPDH localized?

GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus 

(PMIDs: 21895736, 12829261).


Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear 

functions?

Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is 

useful as a control for Western blots and RT-PCR.


What are the known protein-protein interactions of GAPDH?

GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of 

these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).


Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.


Publications

SpeciesApplicationTitle
WB

Mol Cell Biochem

PSAT1 promotes the progression of colorectal cancer by regulating Hippo-YAP/TAZ-ID1 axis via AMOT

Authors - Minshan Tang
View Article