Validation Data Gallery
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Tested Applications
| Positive WB detected in | human plasma |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-68275 targets FC of Human IgG in WB applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg0212 Product name: recombinant human FC of Human IgG protein Source: mammalian cells-derived, pINFUSE-hIgG2-Fc Tag: hIgG2-Fc Sequence: 相同性解析による交差性が予測される生物種 |
| Full Name | FC of Human IgG |
| Observed molecular weight | 55 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| Conjugate | HRP |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Immunoglobulin G (IgG) is one of the most abundant proteins in human serum, accounting for about 10-20% of plasma protein. IgG FC is a homodimer that is composed of the constant region of the two heavy chains that form the IgG molecule. This antibody detects the human IgG FC heavy chain.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP FC of Human IgG antibody HRP-68275 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |