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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using FC of Human IgG Monoclonal antibody (68275-1-Ig)

    WB analysis of human plasma using 68275-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 68275-1-Ig (FC of Human IgG antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Eg0212 using FC of Human IgG Monoclonal antibody (68275-1-Ig)

    WB analysis of Eg0212 using 68275-1-Ig

    Recombinant protein were subjected to SDS PAGE followed by western blot with 68275-1-Ig (FC of Human IgG antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Eg0327 using FC of Human IgG Monoclonal antibody (68275-1-Ig)

    WB analysis of Eg0327 using 68275-1-Ig

    Recombinant protein were subjected to SDS PAGE followed by western blot with 68275-1-Ig (FC of Human IgG antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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FC of Human IgG Monoclonal antibody

FC of Human IgG Monoclonal Antibody for WB, ELISA
Cat No. 68275-1-Ig
Clone No.1A12C11
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, recombinant protein

Applications

WB, ELISA

Synonyms

anti-human IgG Fc, Fc Tag, hFc Tag, IgG Fc

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma, Recombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68275-1-Ig targets FC of Human IgG in WB, ELISA applications and shows reactivity with human, recombinant protein samples.

Tested Reactivity human, recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name FC of Human IgG
Observed molecular weight55 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2935601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Immunoglobulin G (IgG) is one of the most abundant proteins in human serum, accounting for about 10-20% of plasma protein. IgG FC is a homodimer that is composed of the constant region of the two heavy chains that form the IgG molecule. This antibody detects the human IgG FC heavy chain.

Protocols

Product Specific Protocols
WB protocol for FC of Human IgG antibody 68275-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Am J Physiol Endocrinol Metab

Long-lasting recombinant HEXA improves hepatic steatosis and glycemic control in mild, but not severe, metabolic dysfunction-associated steatohepatitis

Authors - Sihan Lin
View Article