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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated EXOSC10 Monoclonal a (CL488-68315)

    IF Staining of HeLa using CL488-68315

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 EXOSC10 antibody (CL488-68315, Clone: 1D6G9 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated EXOSC10 Monoclonal antibody

EXOSC10 Monoclonal Antibody for IF/ICC
Cat No. CL488-68315
Clone No.1D6G9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

RRP6, PM/Scl-100, P100 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component 10, EC:3.1.13.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68315 targets EXOSC10 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28587

Product name: Recombinant human EXOSC10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 586-885 aa of BC039901

Sequence: VAAGVKKSGPLPSAERLENVLFGPHDCSHAPPDGYPIIPTSGSVPVQKQASLFPDEKEDNLLGTTCLIATAVITLFNEPSAEDSKKGPLTVAQKKAQNIMESFENPFRMFLPSLGHRAPVSQAAKFDPSTKIYEISNRWKLAQVQVQKDSKEAVKKKAAEQTAAREQAKEACKAAAEQAISVRQQVVLENAAKKRERATSDPRTTEQKQEKKRLKISKKPKDPEPPEKEFTPYDYSQSDFKAFAGNSKSKVSSQFDPNKQTPSGKKCIAAKKIKQSVGNKSMSFPTGKSDRGFRYNWPQR

相同性解析による交差性が予測される生物種
Full Name exosome component 10
Calculated molecular weight 98 kDa
Observed molecular weight98 kDa
GenBank accession numberBC039901
Gene Symbol EXOSC10
Gene ID (NCBI) 5394
RRIDAB_3672994
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ01780
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EXOSC10 antibody CL488-68315Download protocol
Standard Protocols
Click here to view our Standard Protocols