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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXOSC10 Monoclonal antibody (68315-1-Ig)

    WB analysis using 68315-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68315-1-Ig (EXOSC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Immunoprecipitation (IP) experiment of HeLa cells using EXOSC10 Monoclonal antibody (68315-1-Ig)

    IP experiment of HeLa using 68315-1-Ig

    IP result of anti-EXOSC10 (IP:68315-1-Ig, 4ug; Detection:68315-1-Ig 1:1000) with HeLa cells lysate 1720 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EXOSC10 Monoclonal antibody (68315-1-Ig)

    IF Staining of HeLa using 68315-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EXOSC10 antibody (68315-1-Ig, Clone: 1D6G9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

EXOSC10 Monoclonal antibody

EXOSC10 Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 68315-1-Ig
Clone No.1D6G9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

1D6G9, EC:3.1.13.-, Exosome complex component 10, P100 polymyositis-scleroderma overlap syndrome-associated autoantigen, PM/Scl-100

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68315-1-Ig targets EXOSC10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28587

Product name: Recombinant human EXOSC10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 586-885 aa of BC039901

Sequence: VAAGVKKSGPLPSAERLENVLFGPHDCSHAPPDGYPIIPTSGSVPVQKQASLFPDEKEDNLLGTTCLIATAVITLFNEPSAEDSKKGPLTVAQKKAQNIMESFENPFRMFLPSLGHRAPVSQAAKFDPSTKIYEISNRWKLAQVQVQKDSKEAVKKKAAEQTAAREQAKEACKAAAEQAISVRQQVVLENAAKKRERATSDPRTTEQKQEKKRLKISKKPKDPEPPEKEFTPYDYSQSDFKAFAGNSKSKVSSQFDPNKQTPSGKKCIAAKKIKQSVGNKSMSFPTGKSDRGFRYNWPQR

相同性解析による交差性が予測される生物種
Full Name exosome component 10
Calculated molecular weight 98 kDa
Observed molecular weight98 kDa
GenBank accession numberBC039901
Gene Symbol EXOSC10
Gene ID (NCBI) 5394
RRIDAB_2935392
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ01780
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

Protocols

Product Specific Protocols
WB protocol for EXOSC10 antibody 68315-1-IgDownload protocol
IF protocol for EXOSC10 antibody 68315-1-IgDownload protocol
IP protocol for EXOSC10 antibody 68315-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols