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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated EEA1 Monoclonal antibody

EEA1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E11E4

Cat no : CL594-68065

Synonyms

early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-68065

    IF Staining of HepG2 using CL594-68065

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 EEA1 antibody (CL594-68065, Clone: 1E11E4 ) at dilution of 1:200, CL488-Phalloidin (green).

  • FC experiment of HeLa using CL594-68065

    FC experiment of HeLa using CL594-68065

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EEA1 (CL594-68065, Clone:1E11E4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68065-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-68065 targets EEA1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEA1 fusion protein Ag28814 相同性解析による交差性が予測される生物種
Full Name early endosome antigen 1
Calculated molecular weight 162 kDa
Observed molecular weight 162 kDa
GenBank accession numberNM_003566
Gene symbol EEA1
Gene ID (NCBI) 8411
RRIDAB_2920189
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

Protocols

Product Specific Protocols
IF protocol for CL594 EEA1 antibody CL594-68065Download protocol
FC protocol for CL594 EEA1 antibody CL594-68065Download protocol
Standard Protocols
Click here to view our Standard Protocols