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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EEA1 Monoclonal antibody, PBS Only

EEA1 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11E4

Cat no : 68065-1-PBS

Synonyms

early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2

Validation Data Gallery

Applications:
  • WB analysis using 68065-1-Ig (same clone as 68065-1-PBS)

    WB analysis using 68065-1-Ig (same clone as 68065-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68065-1-Ig (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68065-1-Ig (same clone as 68065-1-PBS)

    IF Staining of HepG2 using 68065-1-Ig (same clone as 68065-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EEA1 antibody (68065-1-Ig, Clone: 1E11E4 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68065-1-Ig (same clone as 68065-1-PBS)

    IF Staining of HeLa using 68065-1-Ig (same clone as 68065-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EEA1 antibody (68065-1-Ig, Clone: 1E11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

68065-1-PBS targets EEA1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEA1 fusion protein Ag28814 相同性解析による交差性が予測される生物種
Full Name early endosome antigen 1
Calculated molecular weight 162 kDa
Observed molecular weight 162 kDa
GenBank accession numberNM_003566
Gene symbol EEA1
Gene ID (NCBI) 8411
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.