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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite® Plus 488-conjugated DUSP22 Monoclonal an (CL488-68032)

    IF Staining of NIH/3T3 using CL488-68032

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 DUSP22 antibody (CL488-68032, Clone: 3B8F1 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated DUSP22 Monoclonal antibody

DUSP22 Monoclonal Antibody for IF/ICC
Cat No. CL488-68032
Clone No.3B8F1

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit

Applications

IF/ICC

Synonyms

DUSP22, FLJ35864, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68032 targets DUSP22 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9831

Product name: Recombinant human DUSP22 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-184 aa of BC022847

Sequence: MGNGMNKILPGLYIGNFKDARDAEQLSKNKVTHILSVHDSARPMLEGVKYLCIPAADSPSQNLTRHFKESIKFIHECRLRGESCLVHCLAGVSRSVTLVIAYIMTVTDFGWEDALHTVRAGRSCANPNVGFQRQLQEFEKHEVHQYRQWLKEEYGESPLQDAEEAKNILAAPGILKFWAFLRRL

相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 22
Calculated molecular weight 184 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC022847
Gene Symbol DUSP22
Gene ID (NCBI) 56940
RRIDAB_3084391
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NRW4
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DUSP22(Dual specificity protein phosphatase 22) is also named as JSP1, LMWDSP2, MKPX. It has been demonstrated to belong to a new subgroup of small DSPs anchored at the membranes by an N-terminal myristic acid moiety and DUSP22 play a regulatory role in the JNK or p38 MAPK pathways(PMID:17384676). It has 2 isoforms produced by alternative splicing with the molecular weight of 21 kDa and 23 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DUSP22 antibody CL488-68032Download protocol
Standard Protocols
Click here to view our Standard Protocols