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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUSP22 Monoclonal antibody (68032-1-Ig)

    WB analysis using 68032-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68032-1-Ig (DUSP22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit brain tissue using DUSP22 Monoclonal antibody (68032-1-Ig)

    WB analysis of rabbit brain using 68032-1-Ig

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68032-1-Ig (DUSP22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using DUSP22 Monoclonal antibody (68032-1-Ig)

    IF Staining of NIH/3T3 using 68032-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using DUSP22 antibody (68032-1-Ig, Clone: 3B8F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

DUSP22 Monoclonal antibody

DUSP22 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68032-1-Ig
Clone No.3B8F1

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

DUSP22, FLJ35864, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat brain tissue, rabbit brain tissue, mouse brain tissue
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68032-1-Ig targets DUSP22 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9831

Product name: Recombinant human DUSP22 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-184 aa of BC022847

Sequence: MGNGMNKILPGLYIGNFKDARDAEQLSKNKVTHILSVHDSARPMLEGVKYLCIPAADSPSQNLTRHFKESIKFIHECRLRGESCLVHCLAGVSRSVTLVIAYIMTVTDFGWEDALHTVRAGRSCANPNVGFQRQLQEFEKHEVHQYRQWLKEEYGESPLQDAEEAKNILAAPGILKFWAFLRRL

相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 22
Calculated molecular weight 184 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC022847
Gene Symbol DUSP22
Gene ID (NCBI) 56940
RRIDAB_2918774
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NRW4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DUSP22(Dual specificity protein phosphatase 22) is also named as JSP1, LMWDSP2, MKPX. It has been demonstrated to belong to a new subgroup of small DSPs anchored at the membranes by an N-terminal myristic acid moiety and DUSP22 play a regulatory role in the JNK or p38 MAPK pathways(PMID:17384676). It has 2 isoforms produced by alternative splicing with the molecular weight of 21 kDa and 23 kDa.

Protocols

Product Specific Protocols
WB protocol for DUSP22 antibody 68032-1-IgDownload protocol
IF protocol for DUSP22 antibody 68032-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols