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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUSP22 Monoclonal antibody (68032-1-Ig)

    WB analysis using 68032-1-Ig (same clone as 68032-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68032-1-Ig (DUSP22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit brain tissue using DUSP22 Monoclonal antibody (68032-1-Ig)

    WB analysis of rabbit brain using 68032-1-Ig (same clone as 68032-1-PBS)

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68032-1-Ig (DUSP22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68032-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using DUSP22 Monoclonal antibody (68032-1-Ig)

    IF Staining of NIH/3T3 using 68032-1-Ig (same clone as 68032-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using DUSP22 antibody (68032-1-Ig, Clone: 3B8F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68032-1-PBS in a different storage buffer formulation.

DUSP22 Monoclonal antibody, PBS Only

DUSP22 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68032-1-PBS
Clone No.3B8F1

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DUSP22, FLJ35864, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68032-1-PBS targets DUSP22 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9831

Product name: Recombinant human DUSP22 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-184 aa of BC022847

Sequence: MGNGMNKILPGLYIGNFKDARDAEQLSKNKVTHILSVHDSARPMLEGVKYLCIPAADSPSQNLTRHFKESIKFIHECRLRGESCLVHCLAGVSRSVTLVIAYIMTVTDFGWEDALHTVRAGRSCANPNVGFQRQLQEFEKHEVHQYRQWLKEEYGESPLQDAEEAKNILAAPGILKFWAFLRRL

相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 22
Calculated molecular weight 184 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC022847
Gene Symbol DUSP22
Gene ID (NCBI) 56940
RRIDAB_2918774
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NRW4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DUSP22(Dual specificity protein phosphatase 22) is also named as JSP1, LMWDSP2, MKPX. It has been demonstrated to belong to a new subgroup of small DSPs anchored at the membranes by an N-terminal myristic acid moiety and DUSP22 play a regulatory role in the JNK or p38 MAPK pathways(PMID:17384676). It has 2 isoforms produced by alternative splicing with the molecular weight of 21 kDa and 23 kDa.