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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated DNAJC18 Polyclonal a (CL488-25162)

    IF Staining of U2OS using CL488-25162

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 DNAJC18 antibody (CL488-25162) at dilution of 1:100.

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated DNAJC18 Polyclonal a (CL488-25162)

    FC experiment of U2OS using CL488-25162

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Dnajc18 Polyclonal Antibody (CL488-25162)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DNAJC18 Polyclonal antibody

DNAJC18 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-25162

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25162 targets DNAJC18 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC18 fusion protein Ag18498 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily C, member 18
Calculated molecular weight 358 aa, 42 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberBC030162
Gene Symbol DNAJC18
Gene ID (NCBI) 202052
RRIDAB_3672785
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9H819
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DnaJC18, also named as DnaJ (Hsp40) homolog, subfamily C, member 21, belongs to Type III DnaJ Family. Type III DnaJ proteins have a J domain but lack the other sequence features that are found in type I and II members of the family. DnaJC18 might play a role in the process of spermatogenesis in adult testis (PMID: 29082339).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DNAJC18 antibody CL488-25162Download protocol
FC protocol for CL Plus 488 DNAJC18 antibody CL488-25162Download protocol
Standard Protocols
Click here to view our Standard Protocols