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DNAJC18 Polyclonal antibody

DNAJC18 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25162-1-AP

Synonyms

DNAJC18

Validation Data Gallery

Applications:
  • WB analysis of A431 using 25162-1-AP

    WB analysis of A431 using 25162-1-AP

    WB result of DNAJC18 antibody (25162-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJC18 transfected A431 cells.

  • WB analysis using 25162-1-AP

    WB analysis using 25162-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25162-1-AP (DNAJC18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 25162-1-AP

    WB analysis using 25162-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25162-1-AP (DNAJC18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 25162-1-AP

    IHC staining of mouse testis using 25162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25162-1-AP (DNAJC18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 25162-1-AP

    IHC staining of mouse testis using 25162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25162-1-AP (DNAJC18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 25162-1-AP

    IF Staining of U2OS using 25162-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DNAJC18 antibody (25162-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U2OS using 25162-1-AP

    IF Staining of U2OS using 25162-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 25162-1-AP (DNAJC18 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

25162-1-AP targets DNAJC18 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC18 fusion protein Ag18498 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily C, member 18
Calculated molecular weight 358 aa, 42 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberBC030162
Gene symbol DNAJC18
Gene ID (NCBI) 202052
RRIDAB_2918087
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DnaJC18, also named as DnaJ (Hsp40) homolog, subfamily C, member 21, belongs to Type III DnaJ Family. Type III DnaJ proteins have a J domain but lack the other sequence features that are found in type I and II members of the family. DnaJC18 might play a role in the process of spermatogenesis in adult testis (PMID: 29082339).

Protocols

Product Specific Protocols
WB protocol for DNAJC18 antibody 25162-1-APDownload protocol
IHC protocol for DNAJC18 antibody 25162-1-APDownload protocol
IF protocol for DNAJC18 antibody 25162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols