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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DHX9 Polyclonal antibody

DHX9 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17721

Synonyms

ATP dependent RNA helicase A, DDX9, DEAH box protein 9, DHX9, LKP, NDH II, NDH2, NDHII, Nuclear DNA helicase II, RHA

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-17721

    IF Staining of HepG2 using CL488-17721

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DHX9 antibody (CL488-17721) at dilution of 1:400.

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DHX9 Ready-To-Use IHC Kit. DHX9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17721-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL488-17721 targets DHX9 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHX9 fusion protein Ag12104 相同性解析による交差性が予測される生物種
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 9
Calculated molecular weight 1270 aa, 141 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC014246
Gene symbol DHX9
Gene ID (NCBI) 1660
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DHX9 antibody CL488-17721Download protocol
Standard Protocols
Click here to view our Standard Protocols