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  • Featured Product
  • KD/KO Validated

DHX9 Polyclonal antibody

DHX9 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 17721-1-AP

Synonyms

ATP dependent RNA helicase A, DDX9, DEAH box protein 9, DHX9, LKP, NDH II, NDH2, NDHII, Nuclear DNA helicase II, RHA

Validation Data Gallery

Applications:
  • WB analysis using 17721-1-AP

    WB analysis using 17721-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 17721-1-AP

    WB analysis using 17721-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis using 17721-1-AP

    WB analysis using 17721-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat spleen using 17721-1-AP

    WB analysis of rat spleen using 17721-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 17721-1-AP (DHX9 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 17721-1-AP

    IHC staining of mouse brain using 17721-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DHX9 antibody included
  • IHC staining of mouse brain using 17721-1-AP

    IHC staining of mouse brain using 17721-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DHX9 antibody included
  • IHC staining of mouse testis using 17721-1-AP

    IHC staining of mouse testis using 17721-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DHX9 antibody included
  • IHC staining of mouse testis using 17721-1-AP

    IHC staining of mouse testis using 17721-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17721-1-AP (DHX9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DHX9 antibody included
  • IF Staining of HepG2 using 17721-1-AP

    IF Staining of HepG2 using 17721-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 17721-1-AP

    IF Staining of HepG2 using 17721-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 17721-1-AP

    IF Staining of HepG2 using 17721-1-AP

    Immunofluorescent analysis of HepG2 cells, using DHX9 antibody 17721-1-AP at 1:100 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IF Staining of HepG2 using 17721-1-AP

    IF Staining of HepG2 using 17721-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DHX9 antibody (17721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, rat spleen tissue, rat testis tissue, Jurkat cells
Positive IHC detected inmouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DHX9 Ready-To-Use IHC Kit. DHX9 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

17721-1-AP targets DHX9 in WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHX9 fusion protein Ag12104 相同性解析による交差性が予測される生物種
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 9
Calculated molecular weight 1270 aa, 141 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC014246
Gene symbol DHX9
Gene ID (NCBI) 1660
RRIDAB_2092506
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].

Protocols

Product Specific Protocols
WB protocol for DHX9 antibody 17721-1-APDownload protocol
IHC protocol for DHX9 antibody 17721-1-APDownload protocol
IF protocol for DHX9 antibody 17721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Hepatology

Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma.

Authors - Zhixiang Hu
View Article
mouseWB

Sci Adv

R-loops coordinate with SOX2 in regulating reprogramming to pluripotency.

Authors - Yaoyi Li
View Article
humanRIP

Nat Commun

Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1.

Authors - Hamed Kooshapur
View Article
humanWB

Nat Commun

Interaction of 7SK with the Smn complex modulates snRNP production.

Authors - Changhe Ji
View Article
humanWB

Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic
View Article
humanWB

Nat Commun

PMID: 31792210

Authors - Sunwang Xu
View Article