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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite® Plus 488-conjugated Calponin Polyclonal (CL488-13938)

    IF Staining of mouse heart using CL488-13938

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite® Plus 488 Calponin antibody (CL488-13938) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using CoraLite® Plus 488-conjugated Calponin Polyclonal (CL488-13938)

    IF Staining of C2C12 using CL488-13938

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite® Plus 488 Calponin antibody (CL488-13938) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite® Plus 488-conjugated Calponin Polyclonal (CL488-13938)

    IF Staining of MDCK using CL488-13938

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 488 Calponin antibody (CL488-13938) at dilution of 1:200.

  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite® Plus 488-conjugated Calponin Polyclonal (CL488-13938)

    FC experiment of C2C12 using CL488-13938

    1x10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Calponin Polyclonal antibody (CL488-13938)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Calponin Polyclonal antibody

Calponin Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL488-13938

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, dog

Applications

IF/ICC, IF-P, FC (Intra)

Synonyms

CNN1, Basic calponin, Calponin 1, Calponin-1, CNN 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inC2C12 cells, MDCK cells
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13938 targets Calponin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, dog samples.

Tested Reactivity human, mouse, rat, dog
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4989

Product name: Recombinant human Calponin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-297 aa of BC022015

Sequence: MSSAHFNRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIGNNFMDGLKDGIILCEFINKLQPGSVKKINESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQSTLLALASMAKTKGNKVNVGVKYAEKQERKFEPGKLREGRNIIGLQMGTNKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQAGMTAPGTKRQIFEPGLGMEHCDTLNVSLQMGSNKGASQRGMTVYGLPRQVYDPKYCLTPEYPELGEPAHNHHAHNYYNSA

相同性解析による交差性が予測される生物種
Full Name calponin 1, basic, smooth muscle
Calculated molecular weight 33 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC022015
Gene Symbol Calponin
Gene ID (NCBI) 1264
RRIDAB_2919081
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP51911
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Calponin antibody CL488-13938Download protocol
FC protocol for CL Plus 488 Calponin antibody CL488-13938Download protocol
Standard Protocols
Click here to view our Standard Protocols