Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | HEK-293 cells |
| Positive FC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-26999 targets CITED1 in FC (Intra) applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24643 Product name: Recombinant human CITED1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-151 aa of BC004240 Sequence: MPTTSRPALDVKGGTSPAKEDANQEMSSVAYSNLAVKDRKAVAILHYPGVASNGTKASGAPTSSSGSPIGSPTTTPPTKPPSFNLHPAPHLLASMQLQKLNSQYQGMAAATPGQPGEAGPLQNWDFGAQAGGAESLSPSAGAQSPAIIDSD 相同性解析による交差性が予測される生物種 |
| Full Name | Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1 |
| Calculated molecular weight | 27 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC004240 |
| Gene Symbol | CITED1 |
| Gene ID (NCBI) | 4435 |
| RRID | AB_2934965 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99966 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CITED1, also named as MSG1, belongs to the CITED family. CITED1 is a bifunctional transcriptional cofactor which is able to activate or repress transcription in association with other transcription factors. CITED1 has 2 isoforms with the molecular mass of 20 and 23 kDa. The elevation of CITED1 may be directly associated with the preneoplastic phenotype (PMID: 23935526).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 CITED1 antibody CL647-26999 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
