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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated CITED1 Polyclonal an (CL488-26999)

    IF Staining of HEK-293 using CL488-26999

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 CITED1 antibody (CL488-26999) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite® Plus 488-conjugated CITED1 Polyclonal an (CL488-26999)

    FC experiment of HEK-293T using CL488-26999

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CITED1 (CL488-26999) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated CITED1 Polyclonal antibody

CITED1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-26999

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

CITED1, Melanocyte specific protein 1, MSG1

Formulation:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293T cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-26999 targets CITED1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24643

Product name: Recombinant human CITED1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-151 aa of BC004240

Sequence: MPTTSRPALDVKGGTSPAKEDANQEMSSVAYSNLAVKDRKAVAILHYPGVASNGTKASGAPTSSSGSPIGSPTTTPPTKPPSFNLHPAPHLLASMQLQKLNSQYQGMAAATPGQPGEAGPLQNWDFGAQAGGAESLSPSAGAQSPAIIDSD

相同性解析による交差性が予測される生物種
Full Name Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
Calculated molecular weight 27 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC004240
Gene Symbol CITED1
Gene ID (NCBI) 4435
RRIDAB_2919211
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99966
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CITED1 antibody CL488-26999Download protocol
Standard Protocols
Click here to view our Standard Protocols
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