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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDKL5 Polyclonal antibody (12973-1-AP)

    WB analysis using 12973-1-AP (same clone as 12973-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12973-1-AP (CDKL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12973-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CDKL5 Polyclonal antibody (12973-1-AP)

    WB analysis using 12973-1-AP (same clone as 12973-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12973-1-AP (CDKL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12973-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CDKL5 Polyclonal antibody (12973-1-AP)

    WB analysis of HepG2 using 12973-1-AP (same clone as 12973-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12973-1-AP (CDKL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12973-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CDKL5 Polyclonal antibody (12973-1-AP)

    IP experiment of mouse brain using 12973-1-AP (same clone as 12973-1-PBS)

    IP result of anti-CDKL5 (IP:12973-1-AP, 4ug; Detection:12973-1-AP 1:300) with mouse brain tissue lysate 3200 ug. This data was developed using the same antibody clone with 12973-1-PBS in a different storage buffer formulation.

CDKL5 Polyclonal antibody, PBS Only

CDKL5 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 12973-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

cyclin dependent kinase like 5, Cyclin-dependent kinase-like 5, EC:2.7.11.22, ISSX, Serine/threonine-protein kinase 9

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12973-1-PBS targets CDKL5 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3531

Product name: Recombinant human CDKL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC036091

Sequence: MKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKFKDSEENEEVKETTLRELKMLRTLKQENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNLSEGNNANYTEYVATRWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQPLFPGESEIDQLFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILNSVLLDLMKNLLKLDPADRYLTEQCLNHPTFQTQR

相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase-like 5
Calculated molecular weight 1030 aa, 114 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC036091
Gene Symbol CDKL5
Gene ID (NCBI) 6792
RRIDAB_2877899
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO76039
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cyclin dependent kinase like 5 (CDKL5) may regulate ciliogenesis and neuronal morphogenesis. It has been reported that CDKL5 is associated with epileptic seizures, severe intellectual disability, and autistic features. The CDKL5 protein is a kinase that regulates key phosphorylation events vital to the development of the complex neuronal network of the brain.

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