CDKL5 Polyclonal antibody

CDKL5 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 12973-1-AP

Synonyms

CDKL5, cyclin dependent kinase like 5, ISSX, STK9



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, PC-12 cells, SH-SY5Y cells, HeLa cell
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12973-1-AP targets CDKL5 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDKL5 fusion protein Ag3531
Full Name cyclin-dependent kinase-like 5
Calculated Molecular Weight 1030 aa, 114 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC036091
Gene Symbol CDKL5
Gene ID (NCBI) 6792
RRIDAB_2877899
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin dependent kinase like 5 (CDKL5) may regulate ciliogenesis and neuronal morphogenesis. It has been reported that CDKL5 is associated with epileptic seizures, severe intellectual disability, and autistic features. The CDKL5 protein is a kinase that regulates key phosphorylation events vital to the development of the complex neuronal network of the brain.

Protocols

Product Specific Protocols
WB protocol for CDKL5 antibody 12973-1-APDownload protocol
IP protocol for CDKL5 antibody 12973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Neurosci Res

Phenotypic characterization of Cdkl5-knockdown neurons establishes elongated cilia as a functional assay for CDKL5 Deficiency Disorder.

Authors - Alessia Di Nardo
humanWB

Cell Rep

A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder

Authors - Hanzhe Kuang