Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, PC-12 cells, SH-SY5Y cells, HeLa cell |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
12973-1-AP targets CDKL5 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3531 Product name: Recombinant human CDKL5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC036091 Sequence: MKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKFKDSEENEEVKETTLRELKMLRTLKQENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNLSEGNNANYTEYVATRWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQPLFPGESEIDQLFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILNSVLLDLMKNLLKLDPADRYLTEQCLNHPTFQTQR 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin-dependent kinase-like 5 |
| Calculated molecular weight | 1030 aa, 114 kDa |
| Observed molecular weight | 100-110 kDa |
| GenBank accession number | BC036091 |
| Gene Symbol | CDKL5 |
| Gene ID (NCBI) | 6792 |
| RRID | AB_2877899 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O76039 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin dependent kinase like 5 (CDKL5) may regulate ciliogenesis and neuronal morphogenesis. It has been reported that CDKL5 is associated with epileptic seizures, severe intellectual disability, and autistic features. The CDKL5 protein is a kinase that regulates key phosphorylation events vital to the development of the complex neuronal network of the brain.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDKL5 antibody 12973-1-AP | Download protocol |
| IP protocol for CDKL5 antibody 12973-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neurosci Res Phenotypic characterization of Cdkl5-knockdown neurons establishes elongated cilia as a functional assay for CDKL5 Deficiency Disorder. | ||
Cell Rep A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder |