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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CALD1 Monoclonal antibody

CALD1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E1C11

Cat no : CL594-66693

Synonyms

CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-66693

    IF Staining of HepG2 using CL594-66693

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66693 (CALD1 antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL594-66693

    FC experiment of HeLa using CL594-66693

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human CALD1 (CL594-66693, Clone:1E1C11) (red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CALD1 Ready-To-Use IHC Kit. CALD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66693-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66693 targets CALD1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CALD1 fusion protein Ag13676 相同性解析による交差性が予測される生物種
Full Name caldesmon 1
Calculated molecular weight 538 aa, 63 kDa
Observed molecular weight 70 kDa~75 kDa
GenBank accession numberBC040354
Gene symbol CALD1
Gene ID (NCBI) 800
RRIDAB_2883614
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 CALD1 antibody CL594-66693Download protocol
Standard Protocols
Click here to view our Standard Protocols