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Tested Applications
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66036 targets ASS1 in IF/ICC applications and shows reactivity with human, pig, mouse, rat samples.
| Tested Reactivity | human, pig, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ASS1 fusion protein Ag9314 相同性解析による交差性が予測される生物種 |
| Full Name | argininosuccinate synthetase 1 |
| Calculated molecular weight | 412 aa, 47 kDa |
| Observed molecular weight | 40-45 kDa |
| GenBank accession number | BC009243 |
| Gene Symbol | ASS1 |
| Gene ID (NCBI) | 445 |
| RRID | AB_2883485 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P00966 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 ASS1 antibody CL594-66036 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |