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- KD/KO Validated
ASS1 Monoclonal antibody
ASS1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig
Applications
WB, IHC, IF
Conjugate
Unconjugated
CloneNo.
1D1A11
Cat no : 66036-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | pig liver tissue, HeLa cells, human liver tissue, L02 cells |
| Positive IHC detected in | human liver tissue, human liver cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy ASS1 Ready-To-Use IHC Kit. ASS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
The immunogen of 66036-1-Ig is ASS1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ASS1 fusion protein Ag9314 相同性解析による交差性が予測される生物種 |
| Full Name | argininosuccinate synthetase 1 |
| Calculated molecular weight | 412 aa, 47 kDa |
| Observed molecular weight | 40-45 kDa |
| GenBank accession number | BC009243 |
| Gene symbol | ASS1 |
| Gene ID (NCBI) | 445 |
| RRID | AB_11042883 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ASS1 antibody 66036-1-Ig | Download protocol |
| IHC protocol for ASS1 antibody 66036-1-Ig | Download protocol |
| IF protocol for ASS1 antibody 66036-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FASEB J Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.
| ||
Mol Pharmacol MicroRNA-1291-5p Sensitizes Pancreatic Carcinoma Cells to Arginine Deprivation and Chemotherapy through the Regulation of Arginolysis and Glycolysis. | ||
BMC Cancer Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy. |
