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Tested Applications
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-84686-4 targets AEG-1/MTDH in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | AEG-1/MTDH fusion protein Ag4840 相同性解析による交差性が予測される生物種 |
| Full Name | metadherin |
| Calculated molecular weight | 582 aa, 64 kDa |
| Observed molecular weight | 75 kDa |
| GenBank accession number | BC045642 |
| Gene Symbol | AEG-1/MTDH |
| Gene ID (NCBI) | 92140 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q86UE4 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AEG-1/MTDH antibody CL488-84686-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |