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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AEG-1/MTDH Recombinant antibody (84686-4-RR)

    WB analysis using 84686-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84686-4-RR (AEG-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AEG-1/MTDH Recombinant antibody (84686-4-RR)

    IF Staining of MCF-7 using 84686-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AEG-1 antibody (84686-4-RR, Clone: 242110B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A549 cells using AEG-1/MTDH Recombinant antibody (84686-4-RR)

    FC experiment of A549 using 84686-4-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug AEG-1/MTDH Recombinant antibody (84686-4-RR, Clone:242110B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84686-4-RR

    Affinity and Kinetic Characterization of 84686-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84686-4-RR against Human AEG-1/MTDH were performed. The affinity constant is below 1 pM.

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AEG-1/MTDH Recombinant antibody

AEG-1/MTDH Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84686-4-RR
Clone No.242110B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

AEG-1, MTDH, 242110B5, 3D3/LYRIC, AEG 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HL-60 cells, MCF-7 cells, HepG2 cells, U-937 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84686-4-RR targets AEG-1/MTDH in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AEG-1/MTDH fusion protein Ag4840 相同性解析による交差性が予測される生物種
Full Name metadherin
Calculated molecular weight 582 aa, 64 kDa
Observed molecular weight75 kDa
GenBank accession numberBC045642
Gene Symbol AEG-1/MTDH
Gene ID (NCBI) 92140
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86UE4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.

Protocols

Product Specific Protocols
WB protocol for AEG-1/MTDH antibody 84686-4-RRDownload protocol
IF protocol for AEG-1/MTDH antibody 84686-4-RRDownload protocol
FC protocol for AEG-1/MTDH antibody 84686-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols