Validation Data Gallery
Tested Applications
| Positive WB detected in | mCherry-Flag/MYC/His Transfected 293T HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-84814 targets 6*his,His tag in WB applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | 6*his,His tag |
| Calculated molecular weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| Conjugate | HRP |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | HISTAG |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP 6*his,His tag antibody HRP-84814 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |