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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using 6*his,His tag Recombinant antibody (84814-1-RR)

    WB analysis using 84814-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84814-1-RR (6*his,His tag antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using 6*his,His tag Recombinant antibody (84814-1-RR)

    IF Staining of HEK-293 using 84814-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 6*his,His tag antibody (84814-1-RR, Clone: 241261A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using 6*his,His tag Recombinant antibody (84814-1-RR)

    FC experiment of Transfected HEK-293T using 84814-1-RR

    1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.25 ug 6*his,His tag Recombinant antibody (84814-1-RR, Clone:241261A7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84814-1-RR

    Affinity and Kinetic Characterization of 84814-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84814-1-RR against a protein with N-terminal 6*His Tag were performed. The affinity constant is 1.69 nM.

  • Affinity and Kinetic Characterization of 84814-1-RR

    Affinity and Kinetic Characterization of 84814-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84814-1-RR against a protein with C-terminal 6*His Tag were performed. The affinity constant is 26.1 nM.

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6*his,His tag Recombinant antibody

6*his,His tag Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84814-1-RR
Clone No.241261A7

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

6*His, His-Tag, 6*His, His Tag, His Tag, 6XHis, 6histag

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTransfected HEK-293T cells
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84814-1-RR targets 6*his,His tag in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name 6*His, His Tag
Calculated molecular weight 1 kDa
Gene Symbol His Tag
Gene ID (NCBI) 5
RRIDAB_3672138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.

Protocols

Product Specific Protocols
WB protocol for 6*his,His tag antibody 84814-1-RRDownload protocol
IF protocol for 6*his,His tag antibody 84814-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols