Tested Applications
| Positive WB detected in | THP-1 cells, HL-60 cells, human placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84827-2-RR targets G-CSFR in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | G-CSFR fusion protein Eg2821 Predict reactive species |
| Full Name | colony stimulating factor 3 receptor (granulocyte) |
| Calculated Molecular Weight | 92 kDa |
| Observed Molecular Weight | 120-140 kDa |
| GenBank Accession Number | NM_000760.3 |
| Gene Symbol | G-CSFR |
| Gene ID (NCBI) | 1441 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q99062-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
G-CSF-R, also named as CSF3R, CD114 and GCSFR is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. It is a 130 kD to 150 kD glycoprotein single chain receptor which binds and activated by GCSF. In addition it may function in some adhesion or recognition events at the cell surface. It has been classified as a member of the hematopoietic (cytokine) receptor family, cytokine receptor class I, or the gp 130 related cytokine receptor family (although it does not apparently bind to gp 130). GCSF receptors can be found on neutrophils, myeloid leukemia cells that respond to GCSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts. In addition, a soluble form is also expressed. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for G-CSFR antibody 84827-2-RR | Download protocol |
| IHC protocol for G-CSFR antibody 84827-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
