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Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using G-CSFR Recombinant antibody (84827-2-RR)

    WB analysis of THP-1 using 84827-2-RR (same clone as 84827-2-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 84827-2-RR (CSF3R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84827-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using G-CSFR Recombinant antibody (84827-2-RR)

    WB analysis using 84827-2-RR (same clone as 84827-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84827-2-RR (CSF3R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84827-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01609-3

    Sandwich ELISA standard curve of MP01609-3

    Sandwich ELISA standard curve of MP01609-3, Human G-CSFR Recombinant Matched Antibody Pair - PBS only. 84827-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2821. 84827-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

  • Sample test of MP01609-3

    Sample test of MP01609-3

    The mean G-CSFR concentration was determined to be 7.76 ng/mL in THP-1 cell extract based on a 4.4 mg/mL extract load.

  • Cytometric bead array standard curve of MP01609-2

    Cytometric bead array standard curve of MP01609-2

    Cytometric bead array standard curve of MP01609-2, G-CSFR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84827-2-PBS. Detection antibody: 84827-3-PBS. Standard: Eg2821. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01609-1

    Cytometric bead array standard curve of MP01609-1

    Cytometric bead array standard curve of MP01609-1, G-CSFR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84827-1-PBS. Detection antibody: 84827-2-PBS. Standard: Eg2821. Range: 0.781-100 ng/mL.

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G-CSFR Recombinant antibody, PBS Only (Capture/Detector)

G-CSFR Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84827-2-PBS
Clone No.242246A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

G-CSF receptor, G CSF receptor, G CSF R, CSF3R, CD114

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84827-2-PBS targets G-CSFR as part of a matched antibody pair:

MP01609-1: 84827-1-PBS capture and 84827-2-PBS detection (validated in Cytometric bead array)

MP01609-2: 84827-2-PBS capture and 84827-3-PBS detection (validated in Cytometric bead array)

MP01609-3: 84827-4-PBS capture and 84827-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 3 receptor (granulocyte)
Calculated molecular weight92 kDa
Observed molecular weight120-140 kDa
GenBank accession numberNM_000760.3
Gene Symbol G-CSFR
Gene ID (NCBI) 1441
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99062-1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

G-CSF-R, also named as CSF3R, CD114 and GCSFR is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. It is a 130 kD to 150 kD glycoprotein single chain receptor which binds and activated by GCSF. In addition it may function in some adhesion or recognition events at the cell surface. It has been classified as a member of the hematopoietic (cytokine) receptor family, cytokine receptor class I, or the gp 130 related cytokine receptor family (although it does not apparently bind to gp 130). GCSF receptors can be found on neutrophils, myeloid leukemia cells that respond to GCSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts. In addition, a soluble form is also expressed. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.