Validation Data Gallery
Filter:
Tested Applications
| Positive IF/ICC detected in | MDA-MB-453 cells |
| Positive FC (Intra) detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82876-1-RR targets SAGE1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag34216 Product name: Recombinant human SAGE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 247-387 aa of NM_018666.2 Sequence: ATITYNVPEEKMEKGQPQPDNILSTASTGLINVAGAGTPAISTNGLYSTVPHNVCEEKMENDQPQPNNVLSTVQPVIIYLTATGIPGMNTRDQYATITHNVCEERVVNNQPLPSNALSTVLPGLAYLATADMPAMSTRDQH 相同性解析による交差性が予測される生物種 |
| Full Name | sarcoma antigen 1 |
| Calculated molecular weight | 99kd |
| GenBank accession number | NM_018666.2 |
| Gene Symbol | SAGE1 |
| Gene ID (NCBI) | 55511 |
| RRID | AB_3670595 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NXZ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SAGE1 antibody 82876-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |