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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated ZP3 Polyclonal antibody

ZP3 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-21279

Synonyms

Sperm receptor, Zona pellucida glycoprotein 3, Zona pellucida protein C, Zp 3, ZP3, ZP3A, ZP3A/ZP3B, ZP3B, ZPC

Validation Data Gallery

Applications:
  • IF Staining of mouse ovary using CL594-21279

    IF Staining of mouse ovary using CL594-21279

    Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using CoraLite®594 ZP3 antibody (CL594-21279) at dilution of 1:200.

  • IF Staining of mouse ovary using CL594-21279

    IF Staining of mouse ovary using CL594-21279

    Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using CoraLite®594 ZP3 antibody (CL594-21279) at dilution of 1:200.

Tested Applications

Positive IF detected inmouse ovary tissue
For other applications, we recommend the unconjugated version of this antibody, 21279-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-21279 is ZP3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZP3 fusion protein Ag15791 相同性解析による交差性が予測される生物種
Full Name zona pellucida glycoprotein 3 (sperm receptor)
Calculated molecular weight 373 aa, 41 kDa
Observed molecular weight 47 kDa, 60 kDa
GenBank accession numberBC113949
Gene symbol ZP3
Gene ID (NCBI) 7784
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that are processed into mature glycoprotein with larger apparent molecular mass (PMID: 8588935; 10341000).

Protocols

Product Specific Protocols
IF protocol for CL594 ZP3 antibody CL594-21279Download protocol
Standard Protocols
Click here to view our Standard Protocols