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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated USP5 Monoclonal antibody

USP5 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F12C10

Cat no : CL488-66213

Synonyms

Deubiquitinating enzyme 5, Isopeptidase T, ISOT, Ubiquitin thiolesterase 5, USP5

Validation Data Gallery

Applications:
  • IF Staining of A549 using CL488-66213

    IF Staining of A549 using CL488-66213

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 USP5 antibody (CL488-66213, Clone: 3F12C10 ) at dilution of 1:400.

Tested Applications

Positive IF detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 66213-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL488-66213 targets USP5 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen USP5 fusion protein Ag7575 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 5 (isopeptidase T)
Calculated molecular weight 96 kDa
Observed molecular weight 95-105 kDa
GenBank accession numberBC005139
Gene symbol USP5
Gene ID (NCBI) 8078
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 USP5 antibody CL488-66213Download protocol
Standard Protocols
Click here to view our Standard Protocols