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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated USP5 Monoclonal antibody

USP5 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F12C10

Cat no : CL488-66213

Synonyms

Deubiquitinating enzyme 5, Isopeptidase T, ISOT, Ubiquitin thiolesterase 5, USP5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated USP5 Monoclonal anti (CL488-66213)

    IF Staining of A549 using CL488-66213

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 USP5 antibody (CL488-66213, Clone: 3F12C10 ) at dilution of 1:400.

  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated USP5 Monoclonal anti (CL488-66213)

    FC experiment of A549 using CL488-66213

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated USP5 Monoclonal antibody (CL488-66213, Clone:3F12C10)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inA549 cells
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66213 targets USP5 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen USP5 fusion protein Ag7575
Full Name ubiquitin specific peptidase 5 (isopeptidase T)
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 95-105 kDa
GenBank Accession NumberBC005139
Gene Symbol USP5
Gene ID (NCBI) 8078
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 USP5 antibody CL488-66213Download protocol
FC protocol for CL Plus 488 USP5 antibody CL488-66213Download protocol
Standard Protocols
Click here to view our Standard Protocols