• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TTLL12 Monoclonal antibody

TTLL12 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H5C11

Cat no : 68044-1-Ig

Synonyms

dJ526I14.2, FLJ41795, KIAA0153, TTLL12

Validation Data Gallery

Applications:
  • WB analysis using 68044-1-Ig

    WB analysis using 68044-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68044-1-Ig (TTLL12 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68044-1-Ig

    IHC staining of human lung cancer using 68044-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68044-1-Ig (TTLL12 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 68044-1-Ig

    IHC staining of human ovary tumor using 68044-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68044-1-Ig (TTLL12 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSKOV-3 cells, LNCap cells, PC-3 cells, A431 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

68044-1-Ig targets TTLL12 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TTLL12 fusion protein Ag13804 相同性解析による交差性が予測される生物種
Full Name tubulin tyrosine ligase-like family, member 12
Calculated molecular weight 644 aa, 74 kDa
Observed molecular weight72-74 kDa
GenBank accession numberBC001070
Gene symbol TTLL12
Gene ID (NCBI) 23170
RRIDAB_2918786
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TTLL12 antibody 68044-1-IgDownload protocol
IHC protocol for TTLL12 antibody 68044-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols