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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated TTLL12 Monoclonal antibody

TTLL12 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H5C11

Cat no : CL488-68044

Synonyms

dJ526I14.2, FLJ41795, KIAA0153, TTLL12

Validation Data Gallery

Applications:
  • FC experiment of A431 using CL488-68044

    FC experiment of A431 using CL488-68044

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TTLL12 (CL488-68044, Clone:1H5C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68044-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-68044 targets TTLL12 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TTLL12 fusion protein Ag13804 相同性解析による交差性が予測される生物種
Full Name tubulin tyrosine ligase-like family, member 12
Calculated molecular weight 644 aa, 74 kDa
Observed molecular weight72-74 kDa
GenBank accession numberBC001070
Gene symbol TTLL12
Gene ID (NCBI) 23170
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 TTLL12 antibody CL488-68044Download protocol
Standard Protocols
Click here to view our Standard Protocols