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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TLE1 Monoclonal antibody

TLE1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F2H11

Cat no : CL488-66216

Synonyms

E(Sp1) homolog, ESG, ESG1, GRG1, TLE1

Validation Data Gallery

Applications:
  • IF Staining of MCF-7 using CL488-66216

    IF Staining of MCF-7 using CL488-66216

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 TLE1 antibody (CL488-66216, Clone: 2F2H11 ) at dilution of 1:200.

Tested Applications

Positive IF detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66216-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66216 targets TLE1 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TLE1 fusion protein Ag21327 相同性解析による交差性が予測される生物種
Full Name transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Calculated molecular weight 83 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC010100
Gene symbol TLE1
Gene ID (NCBI) 7088
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transducin-like enhancer protein 1(TLE1) is a transcriptional corepressor. It can bind to and regress expression of many transcription factors such as NFkB, which is mediated by FOXA2, CTNNB1 and TCF family member in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Expression of TLE1 is highest in skeletal muscle and liver, moderate in placenta, heart, kidney, and spleen, and weak in all other tissues including brain.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TLE1 antibody CL488-66216Download protocol
Standard Protocols
Click here to view our Standard Protocols