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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TGF beta 1 Polyclonal antibody

TGF beta 1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26155-1-AP

Synonyms

TGF beta1, Tgfb, Tgfb 1, Tgfb1, TGF-beta 1, TGFbeta1, TGF-beta1, TGF-β, TGFβ1, TGF-β1

Validation Data Gallery

Applications:
  • WB analysis using 26155-1-AP

    WB analysis using 26155-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 26155-1-AP

    WB analysis of NIH/3T3 using 26155-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 26155-1-AP

    WB analysis using 26155-1-AP

    NIH3T3 cells were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inL-929 cells, NIH/3T3 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

26155-1-AP targets TGF beta 1 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGF beta 1 fusion protein Ag24078 相同性解析による交差性が予測される生物種
Full Name transforming growth factor, beta 1
Observed molecular weight44 kDa
GenBank accession numberNM-011577
Gene symbol Tgfb1
Gene ID (NCBI) 21803
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TGF beta 1 is a potent cytokine capable of regulating multiple cellular processes including cell proliferation, differentiation, wound healing, and immune response. TGF beta 1 is identified in various immune cells and its activity varies in different cell types or cells at different developmental stages1. TGF beta 1 functions by binding to the TGFβ1 receptors, which subsequently activate the canonical SMAD pathway or non-canonical signaling cascades, such as the mitogen-activated protein kinase (MAPK) pathway, phosphatidylinositol-3 kinase and AKT (PI3K-AKT), and Rho family of GTPases (Rho GTPase). What is special about TGF beta 1 is its context-dependent nature that is particularly true for tumors. Full-length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).

Protocols

Product Specific Protocols
WB protocol for TGF beta 1 antibody 26155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols