TGF beta 1 Polyclonal antibody
TGF beta 1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 26155-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L-929 cells, NIH/3T3 cells, RAW 264.7 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
26155-1-AP targets TGF beta 1 in WB, ELISA applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TGF beta 1 fusion protein Ag24078 |
Full Name | transforming growth factor, beta 1 |
Observed Molecular Weight | 44 kDa |
GenBank Accession Number | NM-011577 |
Gene Symbol | Tgfb1 |
Gene ID (NCBI) | 21803 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TGF beta 1 is a potent cytokine capable of regulating multiple cellular processes including cell proliferation, differentiation, wound healing, and immune response. TGF beta 1 is identified in various immune cells and its activity varies in different cell types or cells at different developmental stages1. TGF beta 1 functions by binding to the TGFβ1 receptors, which subsequently activate the canonical SMAD pathway or non-canonical signaling cascades, such as the mitogen-activated protein kinase (MAPK) pathway, phosphatidylinositol-3 kinase and AKT (PI3K-AKT), and Rho family of GTPases (Rho GTPase). What is special about TGF beta 1 is its context-dependent nature that is particularly true for tumors. Full-length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).
Protocols
Product Specific Protocols | |
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WB protocol for TGF beta 1 antibody 26155-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |