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TDP-43 Recombinant antibody
TDP-43 Recombinant Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
16A22
Cat no : 80002-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HAP1, K-562 cells, Neuro-2a cells, C6 cells |
| Positive IP detected in | HAP1 cells |
| Positive IF detected in | SH-SY5Y cells, rat brain tissue, HAP1 cells, HeLa cells, HepG2 cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
80002-1-RR targets TDP-43 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | TDP-43 fusion protein Ag1231 相同性解析による交差性が予測される生物種 |
| Full Name | TAR DNA binding protein |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC001487 |
| Gene symbol | TARDBP |
| Gene ID (NCBI) | 23435 |
| RRID | AB_2882934 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene.
In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).
Proteintech's 80002-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176)
Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274)
80002-1-RR antibody works well in IF experiment.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TDP-43 antibody 80002-1-RR | Download protocol |
| IHC protocol for TDP-43 antibody 80002-1-RR | Download protocol |
| IF protocol for TDP-43 antibody 80002-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neural Regen Res 5-Hydroxytryptamine: a potential therapeutic target in amyotrophic lateral sclerosis | ||
F1000Res The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence | ||
