CoraLite® Plus 488-conjugated Synaptotagmin-1 Monoclonal antibody
Synaptotagmin-1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig, Rabbit, Chicken
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1H1H2
Cat no : CL488-68043
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-68043 targets Synaptotagmin-1 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken samples.
| Tested Reactivity | Human, Mouse, Rat, Pig, Rabbit, Chicken |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Synaptotagmin-1 fusion protein Ag6071 相同性解析による交差性が予測される生物種 |
| Full Name | synaptotagmin I |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 60-65 kDa |
| GenBank accession number | BC058917 |
| Gene symbol | SYT1 |
| Gene ID (NCBI) | 6857 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Synaptotagmin-1 antibody CL488-68043 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
