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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SETD1A Monoclonal antibody

SETD1A Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C2G12

Cat no : CL488-67936

Synonyms

hSET1A, KIAA0339, KMT2F, Lysine N methyltransferase 2F, SET domain containing 1A, Set1, Set1A, SETD1A

Validation Data Gallery

Applications:
  • FC experiment of HEK-293 using CL488-67936

    FC experiment of HEK-293 using CL488-67936

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SETD1A (CL488-67936, Clone:1C2G12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 67936-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67936 targets SETD1A in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SETD1A fusion protein Ag30424 相同性解析による交差性が予測される生物種
Full Name SET domain containing 1A
Calculated molecular weight 186 kDa
Observed molecular weight270 kDa
GenBank accession numberNM_014712
Gene symbol SETD1A
Gene ID (NCBI) 9739
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SETD1A is a SET domain-containing methyltransferase involved in the epigenetic regulation of transcription(PMID:12670868). It is the main catalytic component of a multiprotein complex that methylates lysine 4 of histone H3, a histone mark associated with gene activation (PMID:29937342). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (PMID:31197650). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed:12670868). SETD1A has a calculated molecular weight of 186 kDa, and a 258 kDa band was also reported (PMID: 31076518).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 SETD1A antibody CL488-67936Download protocol
Standard Protocols
Click here to view our Standard Protocols