Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, LNCaP cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
67936-1-Ig targets SETD1A in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30424 Product name: Recombinant human SETD1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-160 aa of NM_014712 Sequence: MDQEGGGDGQKAPSFQWRNYKLIVDPALDPALRRPSQKVYRYDGVHFSVNDSKYIPVEDLQDPRCHVRSKNRDFSLPVPKFKLDEFYIGQIPLKEVTFARLNDNVRETFLKDMCRKYGEVEEVEILLHPRTRKHLGLARVLFTSTRGAKETVKNLHLTSV 相同性解析による交差性が予測される生物種 |
| Full Name | SET domain containing 1A |
| Calculated molecular weight | 186 kDa |
| Observed molecular weight | 270 kDa |
| GenBank accession number | NM_014712 |
| Gene Symbol | SETD1A |
| Gene ID (NCBI) | 9739 |
| RRID | AB_2918688 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O15047 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SETD1A is a SET domain-containing methyltransferase involved in the epigenetic regulation of transcription(PMID:12670868). It is the main catalytic component of a multiprotein complex that methylates lysine 4 of histone H3, a histone mark associated with gene activation (PMID:29937342). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (PMID:31197650). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed:12670868). SETD1A has a calculated molecular weight of 186 kDa, and a 258 kDa band was also reported (PMID: 31076518).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SETD1A antibody 67936-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration | ||
Nat Commun Early-life exercise induces immunometabolic epigenetic modification enhancing anti-inflammatory immunity in middle-aged male mice | ||
J Inflamm (Lond) Histone H3 posttranslational modified enzymes defined neutrophil plasticity and their vulnerability to IL-10 in the course of the inflammation | ||
Cancer Lett PARP1 interacts with WDR5 to enhance target gene recognition and facilitate tumorigenesis | ||
Signal Transduct Target Ther CXXC finger protein 1 (CFP1) bridges the reshaping of genomic H3K4me3 signature to the advancement of lung adenocarcinoma |