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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated SEC24D Monoclonal antibody

SEC24D Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G5G9

Cat no : CL594-67409

Synonyms

FLJ43974, KIAA0755, SEC24 related protein D, SEC24D

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-67409

    IF Staining of HepG2 using CL594-67409

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 SEC24D antibody (CL594-67409, Clone: 3G5G9 ) at dilution of 1:100. DAPI (blue).

Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67409-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67409 targets SEC24D in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SEC24D fusion protein Ag4657 相同性解析による交差性が予測される生物種
Full Name SEC24 family, member D (S. cerevisiae)
Calculated molecular weight 1033 aa, 113 kDa
Observed molecular weight 113 kDa
GenBank accession numberBC035761
Gene symbol SEC24D
Gene ID (NCBI) 9871
RRIDAB_2920127
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

Protocols

Product Specific Protocols
IF protocol for CL594 SEC24D antibody CL594-67409Download protocol
Standard Protocols
Click here to view our Standard Protocols