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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SEC24D Monoclonal antibody (67409-1-Ig)

    WB analysis of Jurkat using 67409-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SEC24D Monoclonal antibody (67409-1-Ig)

    WB analysis of HepG2 using 67409-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SEC24D Monoclonal antibody (67409-1-Ig)

    WB analysis of HeLa using 67409-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SEC24D Monoclonal antibody (67409-1-Ig)

    IF Staining of HepG2 using 67409-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC24D antibody (67409-1-Ig, Clone: 3G5G9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

SEC24D Monoclonal antibody

SEC24D Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67409-1-Ig
Clone No.3G5G9
Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IF/ICC, CoIP, ELISA

Synonyms

SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

CoIPSee 1 publications below

Product Information

67409-1-Ig targets SEC24D in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4657

Product name: Recombinant human SEC24D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 783-1033 aa of BC035761

Sequence: TDALINFFAKSAFKAVLHQPLKVIREILVNQTAHMLACYRKNCASPSAASQLILPDSMKVLPVYMNCLLKNCVLLSRPEISTDERAYQRQLVMTMGVADSQLFFYPQLLPIHTLDVKSTMLPAAVRCSESRLSEEGIFLLANGLHMFLWLGVSSPPELIQGIFNVPSFAHINTDMTLLPEVGNPYSQQLRMIMGIIQQKRPYSMKLTIVKQREQPEMVFRQFLVEDKGLYGGSSYVDFLCCVHKEICQLLN

相同性解析による交差性が予測される生物種
Full Name SEC24 family, member D (S. cerevisiae)
Calculated molecular weight 1033 aa, 113 kDa
Observed molecular weight 113 kDa
GenBank accession numberBC035761
Gene Symbol SEC24D
Gene ID (NCBI) 9871
RRIDAB_2882650
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO94855
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

Protocols

Product Specific Protocols
IF protocol for SEC24D antibody 67409-1-IgDownload protocol
WB protocol for SEC24D antibody 67409-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseCoIP

Cell Death Differ

VMP1 restricts STING trafficking and activation through controlling CDIPT recruitment to ER membrane curvature.

Authors - Chen Zhou
View Article
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