• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated REST Polyclonal antibody

REST Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22242

Synonyms

NRSF, REST, X2 box repressor, XBR

Validation Data Gallery

Applications:
  • IF Staining of A549 using CL488-22242

    IF Staining of A549 using CL488-22242

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 REST antibody (CL488-22242) at dilution of 1:200.

Tested Applications

Positive IF detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy REST Ready-To-Use IHC Kit. REST primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22242-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-22242 targets REST in IF applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REST fusion protein Ag17707 相同性解析による交差性が予測される生物種
Full Name RE1-silencing transcription factor
Calculated molecular weight 1097 aa, 122 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC132859
Gene symbol REST
Gene ID (NCBI) 5978
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 REST antibody CL488-22242Download protocol
Standard Protocols
Click here to view our Standard Protocols